3HEJ

Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature

Summary for 3HEJ

• Entry DOI: 10.2210/pdb3hej/pdb
• Related: 3BDC 3HEH
• Descriptor: Thermonuclease, THYMIDINE-3',5'-DIPHOSPHATE, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (6 entities in total)
• Functional Keywords: staphylococcal nuclease, hyperstable variant, hydrolase, pdtp, domain swapping, arginine, calcium, endonuclease, membrane, metal-binding, nuclease, secreted, zymogen
• Biological source: Staphylococcus aureus
• Cellular location: Nuclease A: Secreted. Nuclease B: Membrane: P00644
• Total number of polymer chains: 4
• Total formula weight: 66827.51

Authors

Khangulov, V.S.,Schlessman, J.L.,Heroux, A.,Garcia-Moreno, E.B. (deposition date: 2009-05-08, release date: 2010-05-19, Last modification date: 2024-02-21)

• Primary citation: Khangulov, V.S.,Schlessman, J.L.,Heroux, A.,Garcia-Moreno, E.B.; Domain swapping promoted by a single mutation that introduces an ionizable group into the hydrophobic core of a protein; To be Published,

Experimental method

X-RAY DIFFRACTION (1.8 Å)