3FW4
Crystal structure of Siderocalin (NGAL, Lipocalin 2) complexed with Ferric Catechol
Summary for 3FW4
| Entry DOI | 10.2210/pdb3fw4/pdb |
| Related | 3FW5 |
| Descriptor | Neutrophil gelatinase-associated lipocalin, FE (III) ION, CATECHOL, ... (7 entities in total) |
| Functional Keywords | 8-stranded anti-parallel beta barrel, 310-helix, glycoprotein, pyrrolidone carboxylic acid, secreted, transport protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Secreted: P80188 |
| Total number of polymer chains | 3 |
| Total formula weight | 63491.67 |
| Authors | Clifton, M.C.,Strong, R.K. (deposition date: 2009-01-16, release date: 2010-05-05, Last modification date: 2024-10-16) |
| Primary citation | Bao, G.,Clifton, M.,Hoette, T.M.,Mori, K.,Deng, S.X.,Qiu, A.,Viltard, M.,Williams, D.,Paragas, N.,Leete, T.,Kulkarni, R.,Li, X.,Lee, B.,Kalandadze, A.,Ratner, A.J.,Pizarro, J.C.,Schmidt-Ott, K.M.,Landry, D.W.,Raymond, K.N.,Strong, R.K.,Barasch, J. Iron traffics in circulation bound to a siderocalin (Ngal)-catechol complex. Nat.Chem.Biol., 6:602-609, 2010 Cited by PubMed Abstract: The lipocalins are secreted proteins that bind small organic molecules. Scn-Ngal (also known as neutrophil gelatinase associated lipocalin, siderocalin, lipocalin 2) sequesters bacterial iron chelators, called siderophores, and consequently blocks bacterial growth. However, Scn-Ngal is also prominently expressed in aseptic diseases, implying that it binds additional ligands and serves additional functions. Using chemical screens, crystallography and fluorescence methods, we report that Scn-Ngal binds iron together with a small metabolic product called catechol. The formation of the complex blocked the reactivity of iron and permitted its transport once introduced into circulation in vivo. Scn-Ngal then recycled its iron in endosomes by a pH-sensitive mechanism. As catechols derive from bacterial and mammalian metabolism of dietary compounds, the Scn-Ngal-catechol-Fe(III) complex represents an unforeseen microbial-host interaction, which mimics Scn-Ngal-siderophore interactions but instead traffics iron in aseptic tissues. These results identify an endogenous siderophore, which may link the disparate roles of Scn-Ngal in different diseases. PubMed: 20581821DOI: 10.1038/nchembio.402 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
Download full validation report
