3FOE

Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases

Summary for 3FOE

• Entry DOI: 10.2210/pdb3foe/pdb
• Related: 2NOV 3FOF
• Descriptor: DNA topoisomerase 4 subunit A, DNA topoisomerase 4 subunit B, DNA (5'-D(P*AP*CP*CP*AP*AP*GP*GP*TP*CP*AP*TP*GP*AP*AP*T)-3'), ... (7 entities in total)
• Functional Keywords: quinolone, topoisomerase, dna, protein-dna cleavage complex, streptococcus pneumoniae, clinafloxacin, cell membrane, dna-binding, isomerase, membrane, atp-binding, nucleotide-binding, isomerase-dna complex, isomerase/dna
• Biological source: Streptococcus pneumoniae; More
• Cellular location: Cell membrane; Peripheral membrane protein (By similarity): P72525
• Total number of polymer chains: 8
• Total formula weight: 195299.44

Authors

Laponogov, I.,Sohi, M.K.,Veselkov, D.A.,Pan, X.-S.,Sawhney, R.,Thompson, A.W.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R. (deposition date: 2008-12-30, release date: 2009-02-24, Last modification date: 2023-11-01)

Primary citation

Laponogov, I.,Sohi, M.K.,Veselkov, D.A.,Pan, X.-S.,Sawhney, R.,Thompson, A.W.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R.
Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases
Nat.Struct.Mol.Biol., 16:667-669, 2009

PubMed Abstract: Type II topoisomerases alter DNA topology by forming a covalent DNA-cleavage complex that allows DNA transport through a double-stranded DNA break. We present the structures of cleavage complexes formed by the Streptococcus pneumoniae ParC breakage-reunion and ParE TOPRIM domains of topoisomerase IV stabilized by moxifloxacin and clinafloxacin, two antipneumococcal fluoroquinolones. These structures reveal two drug molecules intercalated at the highly bent DNA gate and help explain antibacterial quinolone action and resistance.

PubMed: 19448616
DOI: 10.1038/nsmb.1604

Experimental method

X-RAY DIFFRACTION (4.001 Å)