36PO
VIPR Ternary Complex with a duplex dsDNA substrate, 8 subunits
Summary for 36PO
| Entry DOI | 10.2210/pdb36po/pdb |
| EMDB information | 77742 |
| Descriptor | SUSP1 viprRNA, Target DNA, Non-target DNA, ... (4 entities in total) |
| Functional Keywords | ribonucleoprotein, vipr, rna binding protein, ternary complex, rna binding protein-rna-dna complex, rna binding protein/rna/dna |
| Biological source | Suspvirus SUSP1 More |
| Total number of polymer chains | 11 |
| Total formula weight | 253302.21 |
| Authors | Docter, T.A.,Yoon, P.H.,Zhang, Z.,Brohawn, S.G.,Doudna, J.A. (deposition date: 2026-06-24, release date: 2026-09-16, Last modification date: 2026-09-30) |
| Primary citation | Yoon, P.H.,Docter, T.A.,Zhang, Z.T.,Loi, K.,Lopez, S.C.,Valentin-Alvarado, L.E.,Tuck, O.T.,Brohawn, S.G.,Doudna, J.A. VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation. Science, 393:1236-1240, 2026 Cited by PubMed Abstract: Viral interference programmable repeat (VIPR) systems use a noncontiguous code for RNA-guided transcriptional silencing. How the Vipr protein and a VIPR RNA (vrRNA) comprising alternating GGY and NN segments achieve precise DNA targeting is unknown. Here, we present 21 cryo-electron microscopy structures that help explain the mechanism of target engagement. Vipr protomers oligomerize along the vrRNA to form a right-handed helical filament, sequestering each GGY motif and positioning the adjacent NN bases for target base pairing. DNA binding, in which every third nucleotide is skipped, results in a gapped vrRNA-DNA hybrid helix that encircles the nontarget DNA strand to form a geometric triplex. These findings suggest that triplex-mediated target-strand handoff could enable noncontiguous and programmable RNA-guided DNA recognition in VIPR systems. PubMed: 42752121DOI: 10.1126/science.aei3472 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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