31YR

Horse spleen Apoferritin 0.97 MGy variable temperature dose series, 120 K

Summary for 31YR

• Entry DOI: 10.2210/pdb31yr/pdb
• Related: 31XT
• Descriptor: Ferritin light chain, CADMIUM ION (3 entities in total)
• Functional Keywords: metal storage, dose series, temperature series, metal binding protein
• Biological source: Equus caballus (horse)
• Total number of polymer chains: 1
• Total formula weight: 20209.66

Authors

Southworth-Davies, R.J.,Garman, E.F. (deposition date: 2026-06-29, release date: 2026-07-29, Last modification date: 2026-09-30)

Primary citation

Southworth-Davies, R.J.,Murray, J.W.,Carmichael, I.,Rudino-Pinera, E.,Weik, M.,Garman, E.F.
Separating the effects of temperature and absorbed X-ray dose on unit-cell volume.
Acta Crystallogr D Struct Biol, 2026

PubMed Abstract: The behaviour of the unit-cell volume of crystals of the iron-storage molecule ferritin, both in the apo and the holo form, and of influenza A virus subtype N9 neuraminidase at 100 K and over a controlled cryo-temperature series was investigated. The purpose of this study was to separate out the effects of dose and temperature on the protein by assessing the changes in the unit-cell volume. Over the wide range of X-ray doses examined in this work, the irreversible effect of dose on the unit-cell volume could be distinguished from the reversible (below 160 K) temperature-induced effects. Specific structural damage effects were not reversible.

PubMed: 42741972
DOI: 10.1107/S2059798326008612

Experimental method

X-RAY DIFFRACTION (2.15 Å)