2ZZX

Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate

Summary for 2ZZX

• Entry DOI: 10.2210/pdb2zzx/pdb
• Descriptor: ABC transporter, solute-binding protein, LACTIC ACID, PHOSPHATE ION, ... (6 entities in total)
• Functional Keywords: periplasmic substrate binding protein, lactate, trap transporter, transport protein
• Biological source: Thermus thermophilus
• Total number of polymer chains: 4
• Total formula weight: 164022.47

Authors

Akiyama, N.,Takeda, K.,Miki, K. (deposition date: 2009-02-27, release date: 2009-08-11, Last modification date: 2023-11-15)

Primary citation

Akiyama, N.,Takeda, K.,Miki, K.
Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate
J.Mol.Biol., 392:559-565, 2009

PubMed Abstract: Lactate is utilized in many biological processes, and its transport across biological membranes is mediated with various types of transporters. Here, we report the crystal structures of a lactate-binding protein of a TRAP (tripartite ATP-independent periplasmic) secondary transporter from Thermus thermophilus HB8. The folding of the protein is typical for a type II periplasmic solute-binding protein and forms a dimer in a back-to-back manner. One molecule of l-lactate is clearly identified in a cleft of the protein as a complex with a calcium ion. Detailed crystallographic and biochemical analyses revealed that the calcium ion can be removed from the protein and replaced with other divalent cations. This characterization of the structure of a protein binding with calcium lactate makes a significant contribution to our understanding of the mechanisms by which calcium and lactate are accommodated in cells.

PubMed: 19631222
DOI: 10.1016/j.jmb.2009.07.043

Experimental method

X-RAY DIFFRACTION (1.75 Å)