2ZY9

Improved crystal structure of magnesium transporter MgtE

Summary for 2ZY9

• Entry DOI: 10.2210/pdb2zy9/pdb
• Descriptor: Mg2+ transporter MgtE, MAGNESIUM ION (2 entities in total)
• Functional Keywords: membrane protein, metal transport
• Biological source: Thermus thermophilus
• Total number of polymer chains: 2
• Total formula weight: 105298.90

Authors

Nureki, O.,Ishitani, R.,Hattori, M. (deposition date: 2009-01-19, release date: 2009-12-01, Last modification date: 2024-04-03)

Primary citation

Hattori, M.,Iwase, N.,Furuya, N.,Tanaka, Y.,Tsukazaki, T.,Ishitani, R.,Maguire, M.E.,Ito, K.,Maturana, A.,Nureki, O.
Mg(2+)-dependent gating of bacterial MgtE channel underlies Mg(2+) homeostasis
Embo J., 28:3602-3612, 2009

PubMed Abstract: The MgtE family of Mg(2+) transporters is ubiquitously distributed in all phylogenetic domains. Recent crystal structures of the full-length MgtE and of its cytosolic domain in the presence and absence of Mg(2+) suggested a Mg(2+)-homeostasis mechanism, in which the MgtE cytosolic domain acts as a 'Mg(2+) sensor' to regulate the gating of the ion-conducting pore in response to the intracellular Mg(2+) concentration. However, complementary functional analyses to confirm the proposed model have been lacking. Moreover, the limited resolution of the full-length structure precluded an unambiguous characterization of these regulatory divalent-cation-binding sites. Here, we showed that MgtE is a highly Mg(2+)-selective channel gated by Mg(2+) and elucidated the Mg(2+)-dependent gating mechanism of MgtE, using X-ray crystallographic, genetic, biochemical, and electrophysiological analyses. These structural and functional results have clarified the control of Mg(2+) homeostasis through cooperative Mg(2+) binding to the MgtE cytosolic domain.

PubMed: 19798051
DOI: 10.1038/emboj.2009.288

Experimental method

X-RAY DIFFRACTION (2.94 Å)