2ZPP

Neutron crystal structure of cubic insulin at pD9

2ZPP の概要

• エントリーDOI: 10.2210/pdb2zpp/pdb
• 関連するPDBエントリー: 2EFA
• 分子名称: Insulin A chain, Insulin B chain (3 entities in total)
• 機能のキーワード: hormone, cubic, porcine insulin, carbohydrate metabolism, cleavage on pair of basic residues, glucose metabolism, secreted
• 由来する生物種: Sus scrofa (Pig); 詳細
• 細胞内の位置: Secreted: P01315 P01315
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5787.63

構造登録者

Ishikawa, T.,Tanaka, I.,Niimura, N. (登録日: 2008-07-26, 公開日: 2008-10-28, 最終更新日: 2024-10-23)

主引用文献

Ishikawa, T.,Chatake, T.,Morimoto, Y.,Maeda, M.,Kurihara, K.,Tanaka, I.,Niimura, N.
An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis
Biochem.Biophys.Res.Commun., 376:32-35, 2008

PubMed Abstract: Insulin is stored in pancreatic beta-cell as hexameric form with Zn2+ ions, while the hormonally active form is monomer. The hexamer requires the coordination of Zn2+ ions to the HisB10. In order to reveal the mechanism of the hexamerization of insulin, we investigated the Zn2+ free insulin at pD6.6 and pD9 by neutron crystallographic analyses. HisB10 is doubly protonated not only at pD6.6 but also at pD9, indicating an abnormal pK(a) of this histidine. It is suggested that HisB10 acts on a strong cation capture and contributes to the high stability of the hexameric form in pancreas.

PubMed: 18725203
DOI: 10.1016/j.bbrc.2008.08.071

実験手法

NEUTRON DIFFRACTION (2.5 Å)