2ZI8

Crystal structure of the HsaC extradiol dioxygenase from M. tuberculosis in complex with 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (DHSA)

Summary for 2ZI8

• Entry DOI: 10.2210/pdb2zi8/pdb
• Related: 2ZF1
• Descriptor: PROBABLE BIPHENYL-2,3-DIOL 1,2-DIOXYGENASE BPHC, FE (II) ION, 3,4-dihydroxy-9,10-secoandrosta-1(10),2,4-triene-9,17-dione, ... (4 entities in total)
• Functional Keywords: dhsa, hsac, extradiol dioxygenase, mycobacterium tuberculosis, aromatic hydrocarbons catabolism, iron, oxidoreductase
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 2
• Total formula weight: 68001.10

Authors

D'Angelo, I.,Yam, K.C.,Eltis, L.D.,Strynadka, N. (deposition date: 2008-02-13, release date: 2009-02-24, Last modification date: 2023-11-01)

Primary citation

Yam, K.C.,D'Angelo, I.,Kalscheuer, R.,Zhu, H.,Wang, J.X.,Snieckus, V.,Ly, L.H.,Converse, P.J.,Jacobs, W.R.,Strynadka, N.,Eltis, L.D.
Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis.
Plos Pathog., 5:e1000344-e1000344, 2009

PubMed Abstract: Mycobacterium tuberculosis, the etiological agent of TB, possesses a cholesterol catabolic pathway implicated in pathogenesis. This pathway includes an iron-dependent extradiol dioxygenase, HsaC, that cleaves catechols. Immuno-compromised mice infected with a DeltahsaC mutant of M. tuberculosis H37Rv survived 50% longer than mice infected with the wild-type strain. In guinea pigs, the mutant disseminated more slowly to the spleen, persisted less successfully in the lung, and caused little pathology. These data establish that, while cholesterol metabolism by M. tuberculosis appears to be most important during the chronic stage of infection, it begins much earlier and may contribute to the pathogen's dissemination within the host. Purified HsaC efficiently cleaved the catecholic cholesterol metabolite, DHSA (3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione; k(cat)/K(m) = 14.4+/-0.5 microM(-1) s(-1)), and was inactivated by a halogenated substrate analogue (partition coefficient<50). Remarkably, cholesterol caused loss of viability in the DeltahsaC mutant, consistent with catechol toxicity. Structures of HsaC:DHSA binary complexes at 2.1 A revealed two catechol-binding modes: bidentate binding to the active site iron, as has been reported in similar enzymes, and, unexpectedly, monodentate binding. The position of the bicyclo-alkanone moiety of DHSA was very similar in the two binding modes, suggesting that this interaction is a determinant in the initial substrate-binding event. These data provide insights into the binding of catechols by extradiol dioxygenases and facilitate inhibitor design.

PubMed: 19300498
DOI: 10.1371/journal.ppat.1000344

Experimental method

X-RAY DIFFRACTION (2.2 Å)