2ZBO

Crystal structure of low-redox-potential cytochrom c6 from brown alga Hizikia fusiformis at 1.6 A resolution

Summary for 2ZBO

• Entry DOI: 10.2210/pdb2zbo/pdb
• Descriptor: Cytochrome c6, HEME C, SULFATE ION, ... (4 entities in total)
• Functional Keywords: electron transport, cytochrome c6, redox potential, brown alga, heme, iron, metal-binding, transit peptide
• Biological source: Hizikia fusiformis
• Total number of polymer chains: 6
• Total formula weight: 59025.28

Authors

Akazaki, H.,Kawai, F.,Chida, H.,Matsumoto, Y.,Sirasaki, I.,Nakade, H.,Hirayama, M.,Hosikawa, K.,Suruga, K.,Satoh, T.,Yamada, S.,Unzai, S.,Hakamata, W.,Nishio, T.,Park, S.-Y.,Oku, T. (deposition date: 2007-10-26, release date: 2008-09-30, Last modification date: 2025-12-24)

Primary citation

Akazaki, H.,Kawai, F.,Chida, H.,Matsumoto, Y.,Hirayama, M.,Hoshikawa, K.,Unzai, S.,Hakamata, W.,Nishio, T.,Park, S.-Y.,Oku, T.
Cloning, expression and purification of cytochrome c(6) from the brown alga Hizikia fusiformis and complete X-ray diffraction analysis of the structure
ACTA CRYSTALLOGR.,SECT.F, 64:674-680, 2008

PubMed Abstract: The primary sequence of cytochrome c(6) from the brown alga Hizikia fusiformis has been determined by cDNA cloning and the crystal structure has been solved at 1.6 A resolution. The crystal belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 84.58, c = 232.91 A and six molecules per asymmetric unit. The genome code, amino-acid sequence and crystal structure of H. fusiformis cytochrome c(6) were most similar to those of red algal cytochrome c(6). These results support the hypothesis that brown algae acquired their chloroplasts via secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host.

PubMed: 18678931
DOI: 10.1107/S1744309108017752

Experimental method

X-RAY DIFFRACTION (1.6 Å)