2ZBK

Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms

Summary for 2ZBK

• Entry DOI: 10.2210/pdb2zbk/pdb
• Descriptor: Type II DNA topoisomerase VI subunit A, Type 2 DNA topoisomerase 6 subunit B, RADICICOL (3 entities in total)
• Functional Keywords: dna topoisomerase, dna binding protein, decatenation, atpase, drug design, dna-binding, magnesium, metal-binding, atp-binding, nucleotide-binding, structural genomics, paris-sud yeast structural genomics, ysg, isomerase
• Biological source: Sulfolobus shibatae (archaea); More
• Total number of polymer chains: 8
• Total formula weight: 424405.25

Authors

Graille, M.,Cladiere, L.,Durand, D.,Lecointe, F.,Forterre, P.,van Tilbeurgh, H.,Paris-Sud Yeast Structural Genomics (YSG) (deposition date: 2007-10-22, release date: 2008-02-12, Last modification date: 2023-11-01)

Primary citation

Graille, M.,Durand, D.,Lecointe, F.,Gadelle, D.,Quevillon-Cheruel, S.,Vachette, P.,Forterre, P.,van Tilbeurgh, H.
Crystal Structure of an Intact Type II DNA Topoisomerase: Insights into DNA Transfer Mechanisms
Structure, 16:360-370, 2008

PubMed Abstract: DNA topoisomerases resolve DNA topological problems created during transcription, replication, and recombination. These ubiquitous enzymes are essential for cell viability and are highly potent targets for the development of antibacterial and antitumoral drugs. Type II enzymes catalyze the transfer of a DNA duplex through another one in an ATP-dependent mechanism. Because of its small size and sensitivity to antitumoral drugs, the archaeal DNA topoisomerase VI, a type II enzyme, is an excellent model for gaining further understanding of the organization and mechanism of these enzymes. We present the crystal structure of intact DNA topoisomerase VI bound to radicicol, an inhibitor of human topo II, and compare it to the conformation of the apo-protein as determined by small-angle X-ray scattering in solution. This structure, combined with a wealth of experimental data gathered on these enzymes, allows us to propose a structural model for the two-gate DNA transfer mechanism.

PubMed: 18334211
DOI: 10.1016/j.str.2007.12.020

Experimental method

X-RAY DIFFRACTION (3.56 Å)