2ZBK

Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms

2ZBK の概要

• エントリーDOI: 10.2210/pdb2zbk/pdb
• 分子名称: Type II DNA topoisomerase VI subunit A, Type 2 DNA topoisomerase 6 subunit B, RADICICOL (3 entities in total)
• 機能のキーワード: dna topoisomerase, dna binding protein, decatenation, atpase, drug design, dna-binding, magnesium, metal-binding, atp-binding, nucleotide-binding, structural genomics, paris-sud yeast structural genomics, ysg, isomerase
• 由来する生物種: Sulfolobus shibatae (archaea); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 424405.25

構造登録者

Graille, M.,Cladiere, L.,Durand, D.,Lecointe, F.,Forterre, P.,van Tilbeurgh, H.,Paris-Sud Yeast Structural Genomics (YSG) (登録日: 2007-10-22, 公開日: 2008-02-12, 最終更新日: 2023-11-01)

主引用文献

Graille, M.,Durand, D.,Lecointe, F.,Gadelle, D.,Quevillon-Cheruel, S.,Vachette, P.,Forterre, P.,van Tilbeurgh, H.
Crystal Structure of an Intact Type II DNA Topoisomerase: Insights into DNA Transfer Mechanisms
Structure, 16:360-370, 2008

PubMed Abstract: DNA topoisomerases resolve DNA topological problems created during transcription, replication, and recombination. These ubiquitous enzymes are essential for cell viability and are highly potent targets for the development of antibacterial and antitumoral drugs. Type II enzymes catalyze the transfer of a DNA duplex through another one in an ATP-dependent mechanism. Because of its small size and sensitivity to antitumoral drugs, the archaeal DNA topoisomerase VI, a type II enzyme, is an excellent model for gaining further understanding of the organization and mechanism of these enzymes. We present the crystal structure of intact DNA topoisomerase VI bound to radicicol, an inhibitor of human topo II, and compare it to the conformation of the apo-protein as determined by small-angle X-ray scattering in solution. This structure, combined with a wealth of experimental data gathered on these enzymes, allows us to propose a structural model for the two-gate DNA transfer mechanism.

PubMed: 18334211
DOI: 10.1016/j.str.2007.12.020

実験手法

X-RAY DIFFRACTION (3.56 Å)