2ZB9

Crystal structure of TetR family transcription regulator SCO0332

2ZB9 の概要

• エントリーDOI: 10.2210/pdb2zb9/pdb
• 分子名称: Putative transcriptional regulator (2 entities in total)
• 機能のキーワード: transcription regulator, tetr family, helix-turn-helix, dna-binding, transcription regulation, transcription
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47263.46

構造登録者

Okada, U.,Kondo, K.,Watanabe, N.,Yao, M.,Tanaka, I. (登録日: 2007-10-16, 公開日: 2008-01-29, 最終更新日: 2024-03-13)

主引用文献

Okada, U.,Kondo, K.,Hayashi, T.,Watanabe, N.,Yao, M.,Tamura, T.,Tanaka, I.
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor
ACTA CRYSTALLOGR.,SECT.D, 64:198-205, 2008

PubMed Abstract: SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the S atoms of the native protein. SCO0332 contains a helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and forms a homodimer. The overall structure of SCO0332 shows significant similarity to other TetR-family regulators. A systematic evolution of ligands by exponential enrichment (SELEX) analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on the chromosomal DNA of S. coelicolor. An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short-chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.

PubMed: 18219120
DOI: 10.1107/S0907444907059835

実験手法

X-RAY DIFFRACTION (2.25 Å)