2Z4D

NMR Structures of Yeast Proteasome Component Rpn13

2Z4D の概要

• エントリーDOI: 10.2210/pdb2z4d/pdb
• 関連するPDBエントリー: 1FHO 1UBQ 2COC 2D9V 2DHK 2HTH
• 分子名称: 26S proteasome regulatory subunit RPN13 (1 entity in total)
• 機能のキーワード: proteasome, ph domain, nuclear protein
• 由来する生物種: Saccharomyces cerevisiae (yeast)
• 細胞内の位置: Cytoplasm: O13563
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11220.80

構造登録者

Zhang, N.,Walters, K.J. (登録日: 2007-06-15, 公開日: 2008-05-20, 最終更新日: 2024-05-29)

主引用文献

Husnjak, K.,Elsasser, S.,Zhang, N.,Chen, X.,Randles, L.,Shi, Y.,Hofmann, K.,Walters, K.J.,Finley, D.,Dikic, I.
Proteasome subunit Rpn13 is a novel ubiquitin receptor.
Nature, 453:481-488, 2008

PubMed Abstract: Proteasomal receptors that recognize ubiquitin chains attached to substrates are key mediators of selective protein degradation in eukaryotes. Here we report the identification of a new ubiquitin receptor, Rpn13/ARM1, a known component of the proteasome. Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain, which binds K48-linked diubiquitin with an affinity of approximately 90 nM. Like proteasomal ubiquitin receptor Rpn10/S5a, Rpn13 also binds ubiquitin-like (UBL) domains of UBL-ubiquitin-associated (UBA) proteins. In yeast, a synthetic phenotype results when specific mutations of the ubiquitin binding sites of Rpn10 and Rpn13 are combined, indicating functional linkage between these ubiquitin receptors. Because Rpn13 is also the proteasomal receptor for Uch37, a deubiquitinating enzyme, our findings suggest a coupling of chain recognition and disassembly at the proteasome.

PubMed: 18497817
DOI: 10.1038/nature06926

実験手法

SOLUTION NMR