2Z4D
NMR Structures of Yeast Proteasome Component Rpn13
Summary for 2Z4D
| Entry DOI | 10.2210/pdb2z4d/pdb |
| Related | 1FHO 1UBQ 2COC 2D9V 2DHK 2HTH |
| Descriptor | 26S proteasome regulatory subunit RPN13 (1 entity in total) |
| Functional Keywords | proteasome, ph domain, nuclear protein |
| Biological source | Saccharomyces cerevisiae (yeast) |
| Cellular location | Cytoplasm: O13563 |
| Total number of polymer chains | 1 |
| Total formula weight | 11220.80 |
| Authors | Zhang, N.,Walters, K.J. (deposition date: 2007-06-15, release date: 2008-05-20, Last modification date: 2024-05-29) |
| Primary citation | Husnjak, K.,Elsasser, S.,Zhang, N.,Chen, X.,Randles, L.,Shi, Y.,Hofmann, K.,Walters, K.J.,Finley, D.,Dikic, I. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature, 453:481-488, 2008 Cited by PubMed Abstract: Proteasomal receptors that recognize ubiquitin chains attached to substrates are key mediators of selective protein degradation in eukaryotes. Here we report the identification of a new ubiquitin receptor, Rpn13/ARM1, a known component of the proteasome. Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain, which binds K48-linked diubiquitin with an affinity of approximately 90 nM. Like proteasomal ubiquitin receptor Rpn10/S5a, Rpn13 also binds ubiquitin-like (UBL) domains of UBL-ubiquitin-associated (UBA) proteins. In yeast, a synthetic phenotype results when specific mutations of the ubiquitin binding sites of Rpn10 and Rpn13 are combined, indicating functional linkage between these ubiquitin receptors. Because Rpn13 is also the proteasomal receptor for Uch37, a deubiquitinating enzyme, our findings suggest a coupling of chain recognition and disassembly at the proteasome. PubMed: 18497817DOI: 10.1038/nature06926 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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