Crystal structure of the human nuclear membrane zinc metalloprotease ZMPSTE24 mutant (E336A) in complex with a synthetic CSIM tetrapeptide from the C-terminus of prelamin A

Summary for 2YPT

DescriptorCAAX PRENYL PROTEASE 1 HOMOLOG, PRELAMIN-A/C, ZINC ION (3 entities in total)
Functional Keywordshydrolase-peptide complex, m48 peptidase, integral membrane protein, prelamin a processing, ageing, progeria, hydrolase/peptide
Biological sourceHOMO SAPIENS (HUMAN)
Cellular locationEndoplasmic reticulum membrane ; Multi-pass membrane protein  O75844
Nucleus. Isoform C: Nucleus speckle  P02545
Total number of polymer chains8
Total molecular weight224665.88
Primary citation
Quigley, A.,Dong, Y.Y.,Pike, A.C.W.,Dong, L.,Shrestha, L.,Berridge, G.,Stansfeld, P.J.,Sansom, M.S.P.,Edwards, A.M.,Bountra, C.,von Delft, F.,Bullock, A.N.,Burgess-Brown, N.A.,Carpenter, E.P.
The Structural Basis of Zmpste24-Dependent Laminopathies.
Science, 339:1604-, 2013
PubMed: 23539603 (PDB entries with the same primary citation)
DOI: 10.1126/SCIENCE.1231513
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.286102.6%3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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