2YIP

Crystal Structure of Parasite Sarcocystis muris Microneme Protein SML- 2 in complex with 1-Thio-beta-D-Galactose (SPACEGROUP P212121)

2YIP の概要

• エントリーDOI: 10.2210/pdb2yip/pdb
• 関連するPDBエントリー: 2YIL 2YIO
• 分子名称: MICRONEME ANTIGEN L2, 1-thio-beta-D-galactopyranose, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: sugar binding protein, apple-domain tandem repeat, pan_ap, pan_1, galactose-binding lectin, cellular adhesion, micronemal protein
• 由来する生物種: SARCOCYSTIS MURIS
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 92846.90

構造登録者

Mueller, J.J.,Heinemann, U. (登録日: 2011-05-16, 公開日: 2011-11-02, 最終更新日: 2024-10-16)

主引用文献

Mueller, J.J.,Weiss, M.S.,Heinemann, U.
Pan-Modular Structure of Microneme Protein Sml-2 from Parasite Sarcocystis Muris at 1.95 A Resolution and its Complex with 1-Thio-Beta-D-Galactose.
Acta Crystallogr.,Sect.D, D67:936-, 2011

PubMed Abstract: The microneme protein SML-2 is a member of a small family of galactose-specific lectins that play a role during host-cell invasion by the apicomplexan parasite Sarcocystis muris. The structures of apo SML-2 and the 1-thio-β-D-galactose-SML-2 complex were determined at 1.95 and 2.1 Å resolution, respectively, by sulfur-SAD phasing. Highly elongated dimers are formed by PAN-domain tandems in the protomer, bearing the galactose-binding cavities at the distal apple-like domains. The detailed structure of the binding site in SML-2 explains the high specificity of galactose-endgroup binding and the broader specificity of the related Toxoplasma gondii protein TgMIC4 towards galactose and glucose. A large buried surface of highly hydrophobic character and 24 intersubunit hydrogen bonds stabilize the dimers and half of the 12 disulfides per dimer are shielded from the solvent by the polypeptide chain, thereby enhancing the resistance of the parasite protein towards unfolding and proteolysis that allows it to survive within the intestinal tracts of the intermediate and final hosts.

PubMed: 22101820
DOI: 10.1107/S0907444911037796

実験手法

X-RAY DIFFRACTION (2.14 Å)