2YET

Thermoascus GH61 isozyme A

2YET の概要

• エントリーDOI: 10.2210/pdb2yet/pdb
• 関連するPDBエントリー: 3ZUD
• 分子名称: GH61 ISOZYME A, COPPER (II) ION, ACETYL GROUP, ... (6 entities in total)
• 機能のキーワード: hydrolase, degradation of biomass
• 由来する生物種: THERMOASCUS AURANTIACUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49452.69

構造登録者

Otten, H.,Quinlan, R.J.,Sweeney, M.D.,Poulsen, J.-C.N.,Johansen, K.S.,Krogh, K.B.R.M.,Joergensen, C.I.,Tovborg, M.,Anthonsen, A.,Tryfona, T.,Walter, C.P.,Dupree, P.,Xu, F.,Davies, G.J.,Walton, P.H.,Lo Leggio, L. (登録日: 2011-03-30, 公開日: 2011-09-07, 最終更新日: 2023-12-20)

主引用文献

Quinlan, R.J.,Sweeney, M.D.,Lo Leggio, L.,Otten, H.,Poulsen, J.-C.N.,Johansen, K.S.,Krogh, K.B.R.M.,Jorgensen, C.I.,Tovborg, M.,Anthonsen, A.,Tryfona, T.,Walter, C.P.,Dupree, P.,Xu, F.,Davies, G.J.,Walton, P.H.
Insights Into the Oxidative Degradation of Cellulose by a Copper Metalloenzyme that Exploits Biomass Components.
Proc.Natl.Acad.Sci.USA, 108:15079-, 2011

PubMed Abstract: The enzymatic degradation of recalcitrant plant biomass is one of the key industrial challenges of the 21st century. Accordingly, there is a continuing drive to discover new routes to promote polysaccharide degradation. Perhaps the most promising approach involves the application of "cellulase-enhancing factors," such as those from the glycoside hydrolase (CAZy) GH61 family. Here we show that GH61 enzymes are a unique family of copper-dependent oxidases. We demonstrate that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodextrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. By using electron paramagnetic resonance spectroscopy and single-crystal X-ray diffraction, the active site of GH61 is revealed to contain a type II copper and, uniquely, a methylated histidine in the copper's coordination sphere, thus providing an innovative paradigm in bioinorganic enzymatic catalysis.

PubMed: 21876164
DOI: 10.1073/PNAS.1105776108

実験手法

X-RAY DIFFRACTION (1.502 Å)