2YET
Thermoascus GH61 isozyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.404, 88.467, 70.324 |
| Unit cell angles | 90.00, 103.41, 90.00 |
Refinement procedure
| Resolution | 12.333 - 1.502 |
| R-factor | 0.1458 |
| Rwork | 0.144 |
| R-free | 0.18510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vtc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.289 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.330 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 67162 | |
| <I/σ(I)> | 16.3 | 3.3 |
| Completeness [%] | 93.2 | 74.7 |
| Redundancy | 6.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2 M NACL, 0.1 M HEPES, PH 8.0 AND 25%(W/V) PEG 3350. CRYO PROTECTED WITH 8%(W/V) GLYCEROL, 8%(W/V) ETHYLENE GLYCOL, 9%(W/V)D-SUCROSE AND 2%(W/V)D-GLUCOSE |
