Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3ZUD

THERMOASCUS GH61 ISOZYME A

Summary for 3ZUD
Entry DOI10.2210/pdb3zud/pdb
Related2YET
DescriptorGH61 ISOZYME A, COPPER (II) ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
Functional Keywordshydrolase, degradation of recalcitrant biomass
Biological sourceTHERMOASCUS AURANTIACUS
Total number of polymer chains1
Total formula weight25205.34
Authors
Primary citationQuinlan, R.J.,Sweeney, M.D.,Lo Leggio, L.,Otten, H.,Poulsen, J.-C.N.,Johansen, K.S.,Krogh, K.B.R.M.,Jorgensen, C.I.,Tovborg, M.,Anthonsen, A.,Tryfona, T.,Walter, C.P.,Dupree, P.,Xu, F.,Davies, G.J.,Walton, P.H.
Insights Into the Oxidative Degradation of Cellulose by a Copper Metalloenzyme that Exploits Biomass Components.
Proc.Natl.Acad.Sci.USA, 108:15079-, 2011
Cited by
PubMed Abstract: The enzymatic degradation of recalcitrant plant biomass is one of the key industrial challenges of the 21st century. Accordingly, there is a continuing drive to discover new routes to promote polysaccharide degradation. Perhaps the most promising approach involves the application of "cellulase-enhancing factors," such as those from the glycoside hydrolase (CAZy) GH61 family. Here we show that GH61 enzymes are a unique family of copper-dependent oxidases. We demonstrate that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodextrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. By using electron paramagnetic resonance spectroscopy and single-crystal X-ray diffraction, the active site of GH61 is revealed to contain a type II copper and, uniquely, a methylated histidine in the copper's coordination sphere, thus providing an innovative paradigm in bioinorganic enzymatic catalysis.
PubMed: 21876164
DOI: 10.1073/PNAS.1105776108
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.25 Å)
Structure validation

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon