2Y3V

N-terminal head domain of Danio rerio SAS-6

2Y3V の概要

• エントリーDOI: 10.2210/pdb2y3v/pdb
• 関連するPDBエントリー: 2Y3W
• 分子名称: SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG (2 entities in total)
• 機能のキーワード: structural protein, cytoskeleton, basal body, centriole, cartwheel, cartwheel hub
• 由来する生物種: DANIO RERIO (ZEBRAFISH)
• 細胞内の位置: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome : Q7ZVT3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 73095.46

構造登録者

van Breugel, M. (登録日: 2010-12-27, 公開日: 2011-02-09, 最終更新日: 2024-05-01)

主引用文献

van Breugel, M.,Hirono, M.,Andreeva, A.,Yanagisawa, H.A.,Yamaguchi, S.,Nakazawa, Y.,Morgner, N.,Petrovich, M.,Ebong, I.O.,Robinson, C.V.,Johnson, C.M.,Veprintsev, D.,Zuber, B.
Structures of SAS-6 suggest its organization in centrioles.
Science, 331:1196-1199, 2011

PubMed Abstract: Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.

PubMed: 21273447
DOI: 10.1126/science.1199325

実験手法

X-RAY DIFFRACTION (1.92 Å)