2XY5

Crystal structure of an artificial salen-copper basepair in complex with fragment DNA polymerase I from Bacillus stearothermophilus

Summary for 2XY5

• Entry DOI: 10.2210/pdb2xy5/pdb
• Related: 2XO7 2XY6
• Related PRD ID: PRD_900032
• Descriptor: DNA POLYMERASE I, 5'-D(*GP*AP*CP*CP*SAYP*TP*CP*CP*CP*TP)-3', 5'-D(*AP*GP*GP*GP*AP*SAYP*GP*GP*TP*CP)-3', ... (9 entities in total)
• Functional Keywords: transferase-dna complex, synthetic biology, metal basepair, replication, salen complex, transferase/dna
• Biological source: GEOBACILLUS STEAROTHERMOPHILUS; More
• Total number of polymer chains: 3
• Total formula weight: 73332.37

Authors

Kaul, C.,Mueller, M.,Wagner, M.,Schneider, S.,Carell, T. (deposition date: 2010-11-15, release date: 2011-07-27, Last modification date: 2023-12-20)

Primary citation

Kaul, C.,Mueller, M.,Wagner, M.,Schneider, S.,Carell, T.
Reversible Bond Formation Enables the Replication and Amplification of a Crosslinking Salen Complex as an Orthogonal Base Pair.
Nature Chem., 3:794-, 2011

PubMed Abstract: The universal genetic code relies on two hydrogen-bonded Watson-Crick base pairs that can form 64 triplet codons. This places a limit on the number of amino acids that can be encoded, which has motivated efforts to create synthetic base pairs that are orthogonal to the natural ones. An additional base pair would result in another 61 triplet codons. Artificial organic base pairs have been described in enzymatic incorporation studies, and inorganic T-Hg-T and C-Ag-C base pairs have been reported to form in primer extension studies. Here, we demonstrate a metal base pair that is fully orthogonal and can be replicated, and can even be amplified by polymerase chain reaction in the presence of the canonical pairs dA:dT and dG:dC. Crystal structures of a dS-Cu-dS base pair inside a polymerase show that reversible chemistry is possible directly inside the polymerase, which enables the efficient copying of the inorganic crosslink. The results open up the possibility of replicating and amplifying artificial inorganic DNA nanostructures by extending the genetic alphabet.

PubMed: 21941252
DOI: 10.1038/NCHEM.1117

Experimental method

X-RAY DIFFRACTION (2.22 Å)