Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

2XVN

A. fumigatus chitinase A1 phenyl-methylguanylurea complex

Summary for 2XVN
Entry DOI10.2210/pdb2xvn/pdb
Related2XUC 2XVP
DescriptorASPERGILLUS FUMIGATUS CHITINASE A1, 1-METHYL-3-(N-PHENYLCARBAMIMIDOYL)UREA, CHLORIDE ION, ... (4 entities in total)
Functional Keywordshydrolase, inhibitor
Biological sourceASPERGILLUS FUMIGATUS
Total number of polymer chains3
Total formula weight102274.54
Authors
Rush, C.L.,Schuttelkopf, A.W.,Hurtado-Guerrero, R.,Blair, D.E.,Ibrahim, A.F.M.,Desvergnes, S.,Eggleston, I.M.,van Aalten, D.M.F. (deposition date: 2010-10-26, release date: 2010-11-03, Last modification date: 2024-10-23)
Primary citationRush, C.L.,Schuttelkopf, A.W.,Hurtado-Guerrero, R.,Blair, D.E.,Ibrahim, A.F.M.,Desvergnes, S.,Eggleston, I.M.,van Aalten, D.M.F.
Natural Product-Guided Discovery of a Fungal Chitinase Inhibitor.
Chem.Biol., 17:1275-, 2010
Cited by
PubMed Abstract: Natural products are often large, synthetically intractable molecules, yet frequently offer surprising inroads into previously unexplored chemical space for enzyme inhibitors. Argifin is a cyclic pentapeptide that was originally isolated as a fungal natural product. It competitively inhibits family 18 chitinases by mimicking the chitooligosaccharide substrate of these enzymes. Interestingly, argifin is a nanomolar inhibitor of the bacterial-type subfamily of fungal chitinases that possess an extensive chitin-binding groove, but does not inhibit the much smaller, plant-type enzymes from the same family that are involved in fungal cell division and are thought to be potential drug targets. Here we show that a small, highly efficient, argifin-derived, nine-atom fragment is a micromolar inhibitor of the plant-type chitinase ChiA1 from the opportunistic pathogen Aspergillus fumigatus. Evaluation of the binding mode with the first crystal structure of an A. fumigatus plant-type chitinase reveals that the compound binds the catalytic machinery in the same manner as observed for argifin with the bacterial-type chitinases. The structure of the complex was used to guide synthesis of derivatives to explore a pocket near the catalytic machinery. This work provides synthetically tractable plant-type family 18 chitinase inhibitors from the repurposing of a natural product.
PubMed: 21168763
DOI: 10.1016/J.CHEMBIOL.2010.07.018
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.35 Å)
Structure validation

238582

PDB entries from 2025-07-09

PDB statisticsPDBj update infoContact PDBjnumon