2XV4

Structure of Human RPC62 (partial)

2XV4 の概要

• エントリーDOI: 10.2210/pdb2xv4/pdb
• 関連するPDBエントリー: 2XUB
• 分子名称: DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: transcription, winged helix
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus (By similarity): Q9BUI4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60977.47

構造登録者

Lefevre, S.,Legrand, P.,Fribourg, S. (登録日: 2010-10-22, 公開日: 2011-03-02, 最終更新日: 2024-05-08)

主引用文献

Lefevre, S.,Dumay-Odelot, H.,El Ayoubi, L.,Budd, A.,Legrand, P.,Pinaud, N.,Teichmann, M.,Fribourg, S.
Structure-Function Analysis of Hrpc62 Provides Insights Into RNA Polymerase III Transcription
Nat.Struct.Mol.Biol., 18:352-, 2011

PubMed Abstract: The 17-subunit human RNA polymerase III (hPol III) transcribes small, untranslated RNA genes that are involved in the regulation of transcription, splicing and translation. hPol III subunits hRPC62, hRPC39 and hRPC32 form a stable ternary subcomplex required for promoter-specific transcription initiation by hPol III. Here, we report the crystal structure of hRPC62. This subunit folds as a four-tandem extended winged helix (eWH) protein that is structurally related to the transcription factor TFIIEα N terminus. Through biochemical analyses, we mapped the protein-protein interactions of hRPC62, hRPC32 and hRPC39. In addition, we demonstrated that hRPC62 and hRPC39 bind single-stranded and duplex DNA, respectively, in a sequence-independent manner. Overall, we shed light on structural similarities between the hPol III-specific subunit hRPC62 and TFIIEα and propose specific functions for hRPC39 and hRPC62 in transcription initiation by hPol III.

PubMed: 21358628
DOI: 10.1038/NSMB.1996

実験手法

X-RAY DIFFRACTION (2.95 Å)