2XRC

Human complement factor I

2XRC の概要

• エントリーDOI: 10.2210/pdb2xrc/pdb
• 分子名称: HUMAN COMPLEMENT FACTOR I, CALCIUM ION, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: immune system, hydrolase, conglutinogen activating factor, serine protease, complement system
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted, extracellular space: P05156
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 259336.62

構造登録者

Roversi, P.,Johnson, S.,Lea, S.M. (登録日: 2010-09-13, 公開日: 2011-07-13, 最終更新日: 2024-11-20)

主引用文献

Roversi, P.,Johnson, S.,Caesar, J.J.,Mclean, F.,Leath, K.J.,Tsiftsoglou, S.A.,Morgan, B.P.,Harris, C.L.,Sim, R.B.,Lea, S.M.
Structural Basis for Complement Factor I Control and its Disease-Associated Sequence Polymorphisms.
Proc.Natl.Acad.Sci.USA, 108:12839-, 2011

PubMed Abstract: The complement system is a key component of innate and adaptive immune responses. Complement regulation is critical for prevention and control of disease. We have determined the crystal structure of the complement regulatory enzyme human factor I (fI). FI is in a proteolytically inactive form, demonstrating that it circulates in a zymogen-like state despite being fully processed to the mature sequence. Mapping of functional data from mutants of fI onto the structure suggests that this inactive form is maintained by the noncatalytic heavy-chain allosterically modulating activity of the light chain. Once the ternary complex of fI, a cofactor and a substrate is formed, the allosteric inhibition is released, and fI is oriented for cleavage. In addition to explaining how circulating fI is limited to cleaving only C3b/C4b, our model explains the molecular basis of disease-associated polymorphisms in fI and its cofactors.

PubMed: 21768352
DOI: 10.1073/PNAS.1102167108

実験手法

X-RAY DIFFRACTION (2.69 Å)