Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2XNH

Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1

Summary for 2XNH
Entry DOI10.2210/pdb2xnh/pdb
Related1WF6 2XNK
DescriptorDNA TOPOISOMERASE 2-BINDING PROTEIN 1, IODIDE ION (3 entities in total)
Functional Keywordsisomerase, phosphorylation, protein-protein interaction, dna repair
Biological sourceHOMO SAPIENS (HUMAN)
Cellular locationNucleus: Q92547
Total number of polymer chains1
Total formula weight36019.35
Authors
Rappas, M.,Oliver, A.W.,Pearl, L.H. (deposition date: 2010-08-02, release date: 2010-09-01, Last modification date: 2011-07-13)
Primary citationRappas, M.,Oliver, A.W.,Pearl, L.H.
Structure and Function of the Rad9-Binding Region of the DNA-Damage Checkpoint Adaptor Topbp1.
Nucleic Acids Res., 39:313-, 2011
Cited by
PubMed Abstract: TopBP1 is a scaffold protein that coordinates activation of the DNA-damage-checkpoint response by coupling binding of the 9-1-1 checkpoint clamp at sites of ssDNA, to activation of the ATR-ATRIP checkpoint kinase complex. We have now determined the crystal structure of the N-terminal region of human TopBP1, revealing an unexpected triple-BRCT domain structure. The arrangement of the BRCT domains differs significantly from previously described tandem BRCT domain structures, and presents two distinct sites for binding phosphopeptides in the second and third BRCT domains. We show that the site in the second but not third BRCT domain in the N-terminus of TopBP1, provides specific interaction with a phosphorylated motif at pSer387 in Rad9, which can be generated by CK2.
PubMed: 20724438
DOI: 10.1093/NAR/GKQ743
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.8 Å)
Structure validation

227111

건을2024-11-06부터공개중

PDB statisticsPDBj update infoContact PDBjnumon