2XNH
Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1
Summary for 2XNH
Entry DOI | 10.2210/pdb2xnh/pdb |
Related | 1WF6 2XNK |
Descriptor | DNA TOPOISOMERASE 2-BINDING PROTEIN 1, IODIDE ION (3 entities in total) |
Functional Keywords | isomerase, phosphorylation, protein-protein interaction, dna repair |
Biological source | HOMO SAPIENS (HUMAN) |
Cellular location | Nucleus: Q92547 |
Total number of polymer chains | 1 |
Total formula weight | 36019.35 |
Authors | Rappas, M.,Oliver, A.W.,Pearl, L.H. (deposition date: 2010-08-02, release date: 2010-09-01, Last modification date: 2011-07-13) |
Primary citation | Rappas, M.,Oliver, A.W.,Pearl, L.H. Structure and Function of the Rad9-Binding Region of the DNA-Damage Checkpoint Adaptor Topbp1. Nucleic Acids Res., 39:313-, 2011 Cited by PubMed Abstract: TopBP1 is a scaffold protein that coordinates activation of the DNA-damage-checkpoint response by coupling binding of the 9-1-1 checkpoint clamp at sites of ssDNA, to activation of the ATR-ATRIP checkpoint kinase complex. We have now determined the crystal structure of the N-terminal region of human TopBP1, revealing an unexpected triple-BRCT domain structure. The arrangement of the BRCT domains differs significantly from previously described tandem BRCT domain structures, and presents two distinct sites for binding phosphopeptides in the second and third BRCT domains. We show that the site in the second but not third BRCT domain in the N-terminus of TopBP1, provides specific interaction with a phosphorylated motif at pSer387 in Rad9, which can be generated by CK2. PubMed: 20724438DOI: 10.1093/NAR/GKQ743 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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