2XNH
Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 115.770, 115.770, 115.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.263 - 2.800 |
| R-factor | 0.2327 |
| Rwork | 0.232 |
| R-free | 0.25130 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.603 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.770 | 2.950 |
| High resolution limit [Å] | 2.600 | 2.800 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 13035 | |
| <I/σ(I)> | 6.9 | 1.52 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.93 | 6.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 0.1M TRIS-HCL PH 6.8, 0.5M KI, 5% (V/V) GLYCEROL, 22.5% (W/V), 10% PEG 3350 |
