2XNH
Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-20 |
Detector | ADSC CCD |
Spacegroup name | P 21 3 |
Unit cell lengths | 115.770, 115.770, 115.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.263 - 2.800 |
R-factor | 0.2327 |
Rwork | 0.232 |
R-free | 0.25130 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.603 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.770 | 2.950 |
High resolution limit [Å] | 2.600 | 2.800 |
Rmerge | 0.090 | 0.500 |
Number of reflections | 13035 | |
<I/σ(I)> | 6.9 | 1.52 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.93 | 6.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 0.1M TRIS-HCL PH 6.8, 0.5M KI, 5% (V/V) GLYCEROL, 22.5% (W/V), 10% PEG 3350 |