2XNH

Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1

2XNH の概要

• エントリーDOI: 10.2210/pdb2xnh/pdb
• 関連するPDBエントリー: 1WF6 2XNK
• 分子名称: DNA TOPOISOMERASE 2-BINDING PROTEIN 1, IODIDE ION (3 entities in total)
• 機能のキーワード: isomerase, phosphorylation, protein-protein interaction, dna repair
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: Q92547
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36019.35

構造登録者

Rappas, M.,Oliver, A.W.,Pearl, L.H. (登録日: 2010-08-02, 公開日: 2010-09-01, 最終更新日: 2011-07-13)

主引用文献

Rappas, M.,Oliver, A.W.,Pearl, L.H.
Structure and Function of the Rad9-Binding Region of the DNA-Damage Checkpoint Adaptor Topbp1.
Nucleic Acids Res., 39:313-, 2011

PubMed Abstract: TopBP1 is a scaffold protein that coordinates activation of the DNA-damage-checkpoint response by coupling binding of the 9-1-1 checkpoint clamp at sites of ssDNA, to activation of the ATR-ATRIP checkpoint kinase complex. We have now determined the crystal structure of the N-terminal region of human TopBP1, revealing an unexpected triple-BRCT domain structure. The arrangement of the BRCT domains differs significantly from previously described tandem BRCT domain structures, and presents two distinct sites for binding phosphopeptides in the second and third BRCT domains. We show that the site in the second but not third BRCT domain in the N-terminus of TopBP1, provides specific interaction with a phosphorylated motif at pSer387 in Rad9, which can be generated by CK2.

PubMed: 20724438
DOI: 10.1093/NAR/GKQ743

実験手法

X-RAY DIFFRACTION (2.8 Å)