2XKM
Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR
Summary for 2XKM
| Entry DOI | 10.2210/pdb2xkm/pdb |
| Related | 1IFM 1IFN 1PFI 1PJF 1QL1 1QL2 2IFM 2IFN 3IFM 4IFM |
| Descriptor | CAPSID PROTEIN G8P (1 entity in total) |
| Functional Keywords | viral protein, capsid protein, transmembrane, virion, virus coat protein |
| Biological source | PSEUDOMONAS PHAGE PF1 |
| Total number of polymer chains | 1 |
| Total formula weight | 4612.39 |
| Authors | Straus, S.K.,P Scott, W.R.,Schwieters, C.D.,Marvin, D.A. (deposition date: 2010-07-09, release date: 2010-11-24, Last modification date: 2023-12-20) |
| Primary citation | Straus, S.K.,Scott, W.R.,Schwieters, C.D.,Marvin, D.A. Consensus Structure of Pf1 Filamentous Bacteriophage from X-Ray Fibre Diffraction and Solid-State NMR. Eur.Biophys.J., 40:221-, 2011 Cited by PubMed Abstract: Filamentous bacteriophages (filamentous bacterial viruses or Inovirus) are simple and well-characterised macromolecular assemblies that are widely used in molecular biology and biophysics, both as paradigms for studying basic biological questions and as practical tools in areas as diverse as immunology and solid-state physics. The strains fd, M13 and f1 are virtually identical filamentous phages that infect bacteria expressing F-pili, and are sometimes grouped as the Ff phages. For historical reasons fd has often been used for structural studies, but M13 and f1 are more often used for biological experiments. Many other strains have been identified that are genetically quite distinct from Ff and yet have a similar molecular structure and life cycle. One of these, Pf1, gives the highest resolution X-ray fibre diffraction patterns known for filamentous bacteriophage. These diffraction patterns have been used in the past to derive a molecular model for the structure of the phage. Solid-state NMR experiments have been used in separate studies to derive a significantly different model of Pf1. Here we combine previously published X-ray fibre diffraction data and solid-state NMR data to give a consensus structure model for Pf1 filamentous bacteriophage, and we discuss the implications of this model for assembly of the phage at the bacterial membrane. PubMed: 21082179DOI: 10.1007/S00249-010-0640-9 PDB entries with the same primary citation |
| Experimental method | FIBER DIFFRACTION (3.3 Å) SOLID-STATE NMR |
Structure validation
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