2XHE
Crystal structure of the Unc18-syntaxin 1 complex from Monosiga brevicollis
Summary for 2XHE
| Entry DOI | 10.2210/pdb2xhe/pdb |
| Descriptor | UNC18, SYNTAXIN1 (3 entities in total) |
| Functional Keywords | exocytosis, exocytosis complex, snare, neuro fusion, sm protein, choanoflagellates |
| Biological source | MONOSIGA BREVICOLLIS More |
| Total number of polymer chains | 2 |
| Total formula weight | 103977.33 |
| Authors | Burkhardt, P.,Stegmann, C.M.,Wahl, M.C.,Fasshauer, D. (deposition date: 2010-06-14, release date: 2011-06-29, Last modification date: 2023-12-20) |
| Primary citation | Burkhardt, P.,Stegmann, C.M.,Cooper, B.,Kloepper, T.H.,Imig, C.,Varoqueaux, F.,Wahl, M.C.,Fasshauer, D. Primordial Neurosecretory Apparatus Identified in the Choanoflagellate Monosiga Brevicollis. Proc.Natl.Acad.Sci.USA, 108:15264-, 2011 Cited by PubMed Abstract: SNARE protein-driven secretion of neurotransmitters from synaptic vesicles is at the center of neuronal communication. In the absence of the cytosolic protein Munc18-1, synaptic secretion comes to a halt. Although it is believed that Munc18-1 orchestrates SNARE complexes, its mode of action is still a matter of debate. In particular, it has been challenging to clarify the role of a tight Munc18/syntaxin 1 complex, because this interaction interferes strongly with syntaxin's ability to form a SNARE complex. In this complex, two regions of syntaxin, the N-peptide and the remainder in closed conformation, bind to Munc18 simultaneously. Until now, this binary complex has been reported for neuronal tissues only, leading to the hypothesis that it might be a specialization of the neuronal secretion apparatus. Here we aimed, by comparing the core secretion machinery of the unicellular choanoflagellate Monosiga brevicollis with that of animals, to reconstruct the ancestral function of the Munc18/syntaxin1 complex. We found that the Munc18/syntaxin 1 complex from M. brevicollis is structurally and functionally highly similar to the vertebrate complex, suggesting that it constitutes a fundamental step in the reaction pathway toward SNARE assembly. We thus propose that the primordial secretion machinery of the common ancestor of choanoflagellates and animals has been co-opted for synaptic roles during the rise of animals. PubMed: 21876177DOI: 10.1073/PNAS.1106189108 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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