2X0H

BtGH84 Michaelis complex

2X0H の概要

• エントリーDOI: 10.2210/pdb2x0h/pdb
• 関連するPDBエントリー: 2CHN 2CHO 2J47 2J4G 2JIW 2VVN 2VVS 2W4X 2W66 2W67 2WCA 2WZH 2WZI
• 分子名称: O-GLCNACASE BT_4395, 3,4-difluorophenyl 2-deoxy-2-[(difluoroacetyl)amino]-beta-D-glucopyranoside, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: glycoside hydrolase, hydrolase, inhibitor, glycosidase
• 由来する生物種: BACTEROIDES THETAIOTAOMICRON VPI-5482
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 170507.02

構造登録者

He, Y.,Davies, G.J. (登録日: 2009-12-08, 公開日: 2010-01-26, 最終更新日: 2023-12-20)

主引用文献

He, Y.,Macauley, M.S.,Stubbs, K.A.,Vocadlo, D.J.,Davies, G.J.
Visualizing the Reaction Coordinate of an O-Glcnac Hydrolase
J.Am.Chem.Soc., 132:1807-, 2010

PubMed Abstract: N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response, and transcriptional regulation. Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) using a substrate-assisted catalytic mechanism. Here we define the reaction coordinate using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.

PubMed: 20067256
DOI: 10.1021/JA9086769

実験手法

X-RAY DIFFRACTION (2.21 Å)