2X04

Crystal structure of the PABC-TNRC6C complex

2X04 の概要

• エントリーDOI: 10.2210/pdb2x04/pdb
• 関連するPDBエントリー: 1CVJ 1G9L 1JGN 1JH4 2DKL
• 分子名称: POLYADENYLATE-BINDING PROTEIN 1, TRINUCLEOTIDE REPEAT-CONTAINING GENE 6C PROTEIN, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: peptide-rna binding protein complex, rna-mediated gene silencing, nucleus, methylation, spliceosome, translation regulation, protein-protein complex, coiled coil, deadenylation, mrna splicing, phosphoprotein, mrna processing, microrna silencing, peptide/rna binding protein
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm: P11940
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 21566.78

構造登録者

Jinek, M.,Fabian, M.R.,Coyle, S.M.,Sonenberg, N.,Doudna, J.A. (登録日: 2009-12-04, 公開日: 2010-01-19, 最終更新日: 2024-05-08)

主引用文献

Jinek, M.,Fabian, M.R.,Coyle, S.M.,Sonenberg, N.,Doudna, J.A.
Structural Insights Into the Human Gw182-Pabc Interaction in Microrna-Mediated Deadenylation
Nat.Struct.Mol.Biol., 17:238-, 2010

PubMed Abstract: GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.

PubMed: 20098421
DOI: 10.1038/NSMB.1768

実験手法

X-RAY DIFFRACTION (1.49 Å)