2X00

CRYSTAL STRUCTURE OF A-ACHBP IN COMPLEX WITH GYMNODIMINE A

2X00 の概要

• エントリーDOI: 10.2210/pdb2x00/pdb
• 関連するPDBエントリー: 2BR7 2BR8 2BYN 2BYP 2BYQ 2BYR 2BYS 2C9T 2UZ6 2W8E 2W8F 2W8G 2WN9 2WNC 2WNJ 2WNL 2WZY
• 分子名称: SOLUBLE ACETYLCHOLINE RECEPTOR, GYMNODIMINE A, ... (4 entities in total)
• 機能のキーワード: receptor, phycotoxin, toxin, acetylcholine binding protein
• 由来する生物種: APLYSIA CALIFORNICA (CALIFORNIA SEA HARE); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 132258.58

構造登録者

Bourne, Y.,Radic, Z.,Araoz, R.,Talley, T.T.,Benoit, E.,Servent, D.,Taylor, P.,Molgo, J.,Marchot, P. (登録日: 2009-12-04, 公開日: 2010-03-02, 最終更新日: 2024-10-23)

主引用文献

Bourne, Y.,Radic, Z.,Araoz, R.,Talley, T.T.,Benoit, E.,Servent, D.,Taylor, P.,Molgo, J.,Marchot, P.
Structural Determinants in Phycotoxins and Achbp Conferring High Affinity Binding and Nicotinic Achr Antagonism.
Proc.Natl.Acad.Sci.USA, 107:6076-, 2010

PubMed Abstract: Spirolide and gymnodimine macrocyclic imine phycotoxins belong to an emerging class of chemical agents associated with marine algal blooms and shellfish toxicity. Analysis of 13-desmethyl spirolide C and gymnodimine A by binding and voltage-clamp recordings on muscle-type alpha1(2)betagammadelta and neuronal alpha3beta2 and alpha4beta2 nicotinic acetylcholine receptors reveals subnanomolar affinities, potent antagonism, and limited subtype selectivity. Their binding to acetylcholine-binding proteins (AChBP), as soluble receptor surrogates, exhibits picomolar affinities governed by diffusion-limited association and slow dissociation, accounting for apparent irreversibility. Crystal structures of the phycotoxins bound to Aplysia-AChBP ( approximately 2.4A) show toxins neatly imbedded within the nest of ar-omatic side chains contributed by loops C and F on opposing faces of the subunit interface, and which in physiological conditions accommodates acetylcholine. The structures also point to three major features: (i) the sequence-conserved loop C envelops the bound toxins to maximize surface complementarity; (ii) hydrogen bonding of the protonated imine nitrogen in the toxins with the carbonyl oxygen of loop C Trp147 tethers the toxin core centered within the pocket; and (iii) the spirolide bis-spiroacetal or gymnodimine tetrahydrofuran and their common cyclohexene-butyrolactone further anchor the toxins in apical and membrane directions, along the subunit interface. In contrast, the se-quence-variable loop F only sparingly contributes contact points to preserve the broad receptor subtype recognition unique to phycotoxins compared with other nicotinic antagonists. These data offer unique means for detecting spiroimine toxins in shellfish and identify distinctive ligands, functional determinants and binding regions for the design of new drugs able to target several receptor subtypes with high affinity.

PubMed: 20224036
DOI: 10.1073/PNAS.0912372107

実験手法

X-RAY DIFFRACTION (2.4 Å)