2WYY

CRYOEM MODEL OF THE VESICULAR STOMATITIS VIRUS

Summary for 2WYY

• Entry DOI: 10.2210/pdb2wyy/pdb
• Related: 1FZJ 1FZM
• EMDB information: 1663
• Descriptor: NUCLEOPROTEIN, POLY-URIDINE (2 entities in total)
• Functional Keywords: virus, rna, nsrv, helix, virion, viral nucleoprotein
• Biological source: VESICULAR STOMATITIS INDIANA VIRUS (VSV); More
• Total number of polymer chains: 12
• Total formula weight: 502104.49

Authors

Ge, P.,Tsao, J.,Green, T.J.,Luo, M.,Zhou, Z.H. (deposition date: 2009-11-20, release date: 2010-02-16, Last modification date: 2024-05-08)

Primary citation

Ge, P.,Tsao, J.,Schein, S.,Green, T.J.,Luo, M.,Zhou, Z.H.
Cryo-Em Model of the Bullet-Shaped Vesicular Stomatitis Virus.
Science, 327:689-, 2010

PubMed Abstract: Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and a model system of negative-strand RNA viruses. Through direct visualization by means of cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid. Side-by-side interactions between neighboring N subunits are critical for the nucleocapsid to form a bullet shape, and structure-based mutagenesis results support this description. Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer.

PubMed: 20133572
DOI: 10.1126/SCIENCE.1181766

Experimental method

ELECTRON MICROSCOPY (10.6 Å)