2WWZ

TAB2 NZF DOMAIN IN COMPLEX WITH Lys63-linked di-ubiquitin, P212121

2WWZ の概要

• エントリーDOI: 10.2210/pdb2wwz/pdb
• 関連するPDBエントリー: 1AAR 1E0Q 1P3Q 1UZX 1V80 1V81 1WR6 1WRD 1YD8 2C7M 2C7N 2D3G 2FID 2FIF 2WX0 2WX1
• 分子名称: UBIQUITIN, MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-INTERACTING PROTEIN 2, ZINC ION, ... (4 entities in total)
• 機能のキーワード: protein binding, isopeptide bond, nzf domain, zinc-finger, metal-binding
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 20960.27

構造登録者

Kulathu, Y.,Akutsu, M.,Bremm, A.,Hofmann, K.,Komander, D. (登録日: 2009-10-30, 公開日: 2009-11-24, 最終更新日: 2024-05-08)

主引用文献

Kulathu, Y.,Akutsu, M.,Bremm, A.,Hofmann, K.,Komander, D.
Two-Sided Ubiquitin Binding Explains Specificity of the Tab2 Nzf Domain
Nat.Struct.Mol.Biol., 16:1328-, 2009

PubMed Abstract: The protein kinase TAK1 is activated by binding to Lys63 (K63)-linked ubiquitin chains through its subunit TAB2. Here we analyze crystal structures of the TAB2 NZF domain bound to Lys63-linked di- and triubiquitin, revealing that TAB2 binds adjacent ubiquitin moieties via two distinct binding sites. The conformational constraints imposed by TAB2 on a Lys63 dimer cannot be adopted by linear chains, explaining why TAK1 cannot be activated by linear ubiquitination events.

PubMed: 19935683
DOI: 10.1038/NSMB.1731

実験手法

X-RAY DIFFRACTION (1.4 Å)