2WSV

Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with lactose

Summary for 2WSV

• Entry DOI: 10.2210/pdb2wsv/pdb
• Related: 2WS0 2WST 2WSU 2WT1 2WT2
• Related PRD ID: PRD_900008
• Descriptor: PUTATIVE FIBER PROTEIN, beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, NITRATE ION, ... (4 entities in total)
• Functional Keywords: tandem-repeat-type, viral protein, carbohydrate recognition domain
• Biological source: PORCINE ADENOVIRUS 4
• Total number of polymer chains: 1
• Total formula weight: 38717.76

Authors

Guardado-Calvo, P.,Munoz, E.M.,Llamas-Saiz, A.L.,Fox, G.C.,Glasgow, J.N.,van Raaij, M.J. (deposition date: 2009-09-10, release date: 2010-08-11, Last modification date: 2023-12-20)

Primary citation

Guardado-Calvo, P.,Munoz, E.M.,Llamas-Saiz, A.L.,Fox, G.C.,Kahn, R.,Curiel, D.T.,Glasgow, J.N.,van Raaij, M.J.
Crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains.
J. Virol., 84:10558-10568, 2010

PubMed Abstract: Adenovirus isolate NADC-1, a strain of porcine adenovirus type 4, has a fiber containing an N-terminal virus attachment region, shaft and head domains, and a C-terminal galectin domain connected to the head by an RGD-containing sequence. The crystal structure of the head domain is similar to previously solved adenovirus fiber head domains, but specific residues for binding the coxsackievirus and adenovirus receptor (CAR), CD46, or sialic acid are not conserved. The structure of the galectin domain reveals an interaction interface between its two carbohydrate recognition domains, locating both sugar binding sites face to face. Sequence evidence suggests other tandem-repeat galectins have the same arrangement. We show that the galectin domain binds carbohydrates containing lactose and N-acetyl-lactosamine units, and we present structures of the galectin domain with lactose, N-acetyl-lactosamine, 3-aminopropyl-lacto-N-neotetraose, and 2-aminoethyl-tri(N-acetyl-lactosamine), confirming the domain as a bona fide galectin domain.

PubMed: 20686025
DOI: 10.1128/JVI.00997-10

Experimental method

X-RAY DIFFRACTION (2 Å)