2WKJ

Crystal structure of the E192N mutant of E. Coli N-acetylneuraminic acid lyase in complex with pyruvate at 1.45A resolution in space group P212121

Summary for 2WKJ

Entry DOI	10.2210/pdb2wkj/pdb
Related	1FDY 1FDZ 1HL2 1NAL
Descriptor	N-ACETYLNEURAMINATE LYASE, PENTAETHYLENE GLYCOL, PYRUVIC ACID, ... (4 entities in total)
Functional Keywords	directed evolution, sialic acid mimetics, lyase, aldolase, schiff base, carbohydrate metabolism, n-acetylneuraminic acid lyase
Biological source	ESCHERICHIA COLI
Total number of polymer chains	4
Total formula weight	134978.06
Authors	Campeotto, I.,Carr, S.B.,Trinh, C.H.,Nelson, A.S.,Berry, A.,Phillips, S.E.V.,Pearson, A.R. (deposition date: 2009-06-11, release date: 2009-12-01, Last modification date: 2023-12-13)
Primary citation	Campeotto, I.,Carr, S.B.,Trinh, C.H.,Nelson, A.S.,Berry, A.,Phillips, S.E.,Pearson, A.R. Structure of an Escherichia coli N-acetyl-D-neuraminic acid lyase mutant, E192N, in complex with pyruvate at 1.45 angstrom resolution. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 65:1088-1090, 2009 Cited by PubMed: 19923724 DOI: 10.1107/S1744309109037403 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.45 Å)

Structure validation