2WC1

Three-dimensional Structure of the Nitrogen Fixation Flavodoxin (NifF) from Rhodobacter capsulatus at 2.2 A

2WC1 の概要

• エントリーDOI: 10.2210/pdb2wc1/pdb
• 分子名称: FLAVODOXIN, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: electron transport, flavoprotein
• 由来する生物種: RHODOBACTER CAPSULATUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20290.75

構造登録者

Perez-Dorado, I.,Bittel, C.,Hermoso, J.A.,Cortez, N.,Carrillo, N. (登録日: 2009-03-06, 公開日: 2010-04-21, 最終更新日: 2023-12-13)

主引用文献

Perez-Dorado, I.,Bortolotti, A.,Cortez, N.,Hermoso, J.A.
Structural and Phylogenetic Analysis of Rhodobacter Capsulatus Niff: Uncovering General Features of Nitrogen-Fixation (Nif)-Flavodoxins.
Int.J.Mol.Sci., 14:1152-, 2013

PubMed Abstract: Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50's loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as "short-chain" with the firmicutes flavodoxins and the "long-chain" with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.

PubMed: 23303276
DOI: 10.3390/IJMS14011152

実験手法

X-RAY DIFFRACTION (2.17 Å)