2WBU

CRYSTAL STRUCTURE OF THE ZINC FINGER DOMAIN OF KLF4 BOUND TO ITS TARGET DNA

2WBU の概要

• エントリーDOI: 10.2210/pdb2wbu/pdb
• 関連するPDBエントリー: 2WBS
• 分子名称: KRUEPPEL-LIKE FACTOR 4, 5'-D(*DGP*DAP*DGP*DGP*DCP*DGP*DTP* DGP*DGP*DC)-3', 5'-D(*DGP*DCP*DCP*DAP*DCP*DGP*DCP* DCP*DTP*DC)-3', ... (5 entities in total)
• 機能のキーワード: transcription-dna complex, nucleus, activator, dna-binding, zinc-finger, transcription, metal-binding, transcription regulation, transcription/dna
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE)
• 細胞内の位置: Nucleus: Q60793
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 16861.15

構造登録者

Schuetz, A.,Zocher, G.,Carstanjen, D.,Heinemann, U. (登録日: 2009-03-05, 公開日: 2010-04-07, 最終更新日: 2023-12-13)

主引用文献

Schuetz, A.,Nana, D.,Rose, C.,Zocher, G.,Milanovic, M.,Koenigsmann, J.,Blasig, R.,Heinemann, U.,Carstanjen, D.
The Structure of the Klf4 DNA-Binding Domain Links to Self-Renewal and Macrophage Differentiation.
Cell.Mol.Life Sci., 68:3121-, 2011

PubMed Abstract: Krueppel-like factor 4 (Klf4) belongs to the Sp/Klf family of zinc-finger transcription factors and is indispensable for terminal maturation of epithelial tissues. Furthermore, it is part of a small set of proteins that are used to generate pluripotent embryonic stem cells from differentiated tissues. Herein, we describe that a Klf4 zinc-finger domain mutant induces self-renewal and block of maturation, while wild-type Klf4 induces terminal macrophage differentiation. Moreover, we present the crystal structure of the zinc-finger domain of Klf4 bound to its target DNA, revealing that primarily the two C-terminal zinc-finger motifs are required for site specificity. Lack of those two zinc fingers leads to deficiency of Klf4 to induce macrophage differentiation. The first zinc finger, on the other hand, inhibits the otherwise cryptic self-renewal and block of differentiation activity of Klf4. Our data show that impairing the DNA binding could potentially contribute to a monocytic leukemia.

PubMed: 21290164
DOI: 10.1007/S00018-010-0618-X

実験手法

X-RAY DIFFRACTION (2.5 Å)