2WB1

The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase

2WB1 の概要

• エントリーDOI: 10.2210/pdb2wb1/pdb
• 関連するPDBエントリー: 2WAQ
• 分子名称: DNA-DIRECTED RNA POLYMERASE RPO1N SUBUNIT, DNA-DIRECTED RNA POLYMERASE RPO13 SUBUNIT, DNA-DIRECTED RNA POLYMERASE RPO11 SUBUNIT, ... (16 entities in total)
• 機能のキーワード: rna-polymerase, multi-subunit enzyme, transcription
• 由来する生物種: SULFOLOBUS SHIBATAE; 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 812671.38

構造登録者

Korkhin, Y.,Unligil, U.M.,Littlefield, O.,Nelson, P.J.,Stuart, D.I.,Sigler, P.B.,Bell, S.D.,Abrescia, N.G.A. (登録日: 2009-02-19, 公開日: 2009-05-19, 最終更新日: 2023-12-13)

主引用文献

Korkhin, Y.,Unligil, U.M.,Littlefield, O.,Nelson, P.J.,Stuart, D.I.,Sigler, P.B.,Bell, S.D.,Abrescia, N.G.A.
Evolution of Complex RNA Polymerase: The Complete Archaeal RNA Polymerase Structure
Plos Biol., 7:E102-, 2009

PubMed Abstract: The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.

PubMed: 19419240
DOI: 10.1371/JOURNAL.PBIO.1000102

実験手法

X-RAY DIFFRACTION (3.52 Å)