2WB1

The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase

Summary for 2WB1

• Entry DOI: 10.2210/pdb2wb1/pdb
• Related: 2WAQ
• Descriptor: DNA-DIRECTED RNA POLYMERASE RPO1N SUBUNIT, DNA-DIRECTED RNA POLYMERASE RPO13 SUBUNIT, DNA-DIRECTED RNA POLYMERASE RPO11 SUBUNIT, ... (16 entities in total)
• Functional Keywords: rna-polymerase, multi-subunit enzyme, transcription
• Biological source: SULFOLOBUS SHIBATAE; More
• Total number of polymer chains: 26
• Total formula weight: 812671.38

Authors

Korkhin, Y.,Unligil, U.M.,Littlefield, O.,Nelson, P.J.,Stuart, D.I.,Sigler, P.B.,Bell, S.D.,Abrescia, N.G.A. (deposition date: 2009-02-19, release date: 2009-05-19, Last modification date: 2024-11-06)

Primary citation

Korkhin, Y.,Unligil, U.M.,Littlefield, O.,Nelson, P.J.,Stuart, D.I.,Sigler, P.B.,Bell, S.D.,Abrescia, N.G.A.
Evolution of Complex RNA Polymerase: The Complete Archaeal RNA Polymerase Structure
Plos Biol., 7:E102-, 2009

PubMed Abstract: The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.

PubMed: 19419240
DOI: 10.1371/JOURNAL.PBIO.1000102

Experimental method

X-RAY DIFFRACTION (3.52 Å)