2WA0

Crystal structure of the human MAGEA4

2WA0 の概要

• エントリーDOI: 10.2210/pdb2wa0/pdb
• 関連するPDBエントリー: 1I4F
• 分子名称: MELANOMA-ASSOCIATED ANTIGEN 4 (2 entities in total)
• 機能のキーワード: immune system, multiple anomalous dispersion, cancer/testis antigen 1.4, melanoma antigen family a 4, ct1.4, mage-41, mage-x2, tumor antigen, melanoma-associated antigen 4
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27415.18

構造登録者

Roos, A.K.,Cooper, C.D.O.,Ugochukwu, E.,W Yue, W.,Berridge, G.,Elkins, J.M.,Pike, A.C.W.,Bray, J.,Filippakopoulos, P.,Muniz, J.,Chaikuad, A.,Burgess-Brown, N.,Arrowsmith, C.H.,Weigelt, J.,Edwards, A.,Bountra, C.,von Delft, F.,Gileadi, O.,Oppermann, U. (登録日: 2009-01-31, 公開日: 2009-03-10, 最終更新日: 2024-05-08)

主引用文献

Newman, J.A.,Cooper, C.D.O.,Roos, A.K.,Aitkenhead, H.,Oppermann, U.C.T.,Cho, H.J.,Osman, R.,Gileadi, O.
Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.
Plos One, 11:48762-, 2016

PubMed Abstract: The MAGE (melanoma associated antigen) protein family are tumour-associated proteins normally present only in reproductive tissues such as germ cells of the testis. The human genome encodes over 60 MAGE genes of which one class (containing MAGE-A3 and MAGE-A4) are exclusively expressed in tumours, making them an attractive target for the development of targeted and immunotherapeutic cancer treatments. Some MAGE proteins are thought to play an active role in driving cancer, modulating the activity of E3 ubiquitin ligases on targets related to apoptosis. Here we determined the crystal structures of MAGE-A3 and MAGE-A4. Both proteins crystallized with a terminal peptide bound in a deep cleft between two tandem-arranged winged helix domains. MAGE-A3 (but not MAGE-A4), is predominantly dimeric in solution. Comparison of MAGE-A3 and MAGE-A3 with a structure of an effector-bound MAGE-G1 suggests that a major conformational rearrangement is required for binding, and that this conformational plasticity may be targeted by allosteric binders.

PubMed: 26910052
DOI: 10.1371/JOURNAL.PONE.0148762

実験手法

X-RAY DIFFRACTION (2.3 Å)