2W3T

Chloro complex of the Ni-Form of E.coli deformylase

2W3T の概要

• エントリーDOI: 10.2210/pdb2w3t/pdb
• 関連するPDBエントリー: 1BS4 1BS5 1BS6 1BS7 1BS8 1BSJ 1BSK 1BSZ 1DEF 1DFF 1DTF 1G27 1G2A 1ICJ 1LRU 2AI8 2DEF 2DTF 2VHM 2W3U
• 分子名称: PEPTIDE DEFORMYLASE, NICKEL (II) ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: protein biosynthesis, iron, nickel, hydrolase, metal-binding
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21579.86

構造登録者

Ngo, Y.H.T.,Palm, G.J.,Hinrichs, W. (登録日: 2008-11-14, 公開日: 2009-12-15, 最終更新日: 2023-12-13)

主引用文献

Yen, N.T.H.,Bogdanovic, X.,Palm, G.J.,Kuhl, O.,Hinrichs, W.
Structure of the Ni(II) Complex of Escherichia Coli Peptide Deformylase and Suggestions on Deformylase Activities Depending on Different Metal(II) Centres.
J.Biol.Inorg.Chem., 15:195-, 2010

PubMed Abstract: Crystal structures of polypeptide deformylase (PDF) of Escherichia coli with nickel(II) replacing the native iron(II) have been solved with chloride and formate as metal ligands. The chloro complex is a model for the correct protonation state of the hydrolytic hydroxo ligand and the protonated status of the Glu133 side chain as part of the hydrolytic mechanism. The ambiguity that recently some PDFs have been identified with Zn(2+) ion as the active-site centre whereas others are only active with Fe(2+) (or Co(2+), Ni(2+) is discussed with respect to Lewis acid criteria of the metal ion and substrate activation by the CD loop.

PubMed: 20112455
DOI: 10.1007/S00775-009-0583-8

実験手法

X-RAY DIFFRACTION (1.69 Å)