2W0C

X-ray structure of the entire lipid-containing bacteriophage PM2

Summary for 2W0C

• Entry DOI: 10.2210/pdb2w0c/pdb
• Related: 2VVD 2VVE 2VVF
• Descriptor: MAJOR CAPSID PROTEIN P2, PROTEIN 2, PROTEIN P3, ... (5 entities in total)
• Functional Keywords: virus, member of prd1-adeno viral lineage, membrane-containing bacteriophage, virus virion, membrane, transmembrane, capsid protein
• Biological source: PSEUDOALTEROMONAS PHAGE PM2; More
• Cellular location: Virion : P15794 Q9XJR3; Virion membrane ; Single- pass membrane protein : Q9XJR6; Virion membrane : Q9XJR1
• Total number of polymer chains: 16
• Total formula weight: 397751.50

Authors

Abrescia, N.G.A.,Grimes, J.M.,Kivela, H.M.,Assenberg, R.,Sutton, G.C.,Butcher, S.J.,Bamford, J.K.H.,Bamford, D.H.,Stuart, D.I. (deposition date: 2008-08-13, release date: 2008-09-23, Last modification date: 2024-05-08)

Primary citation

Abrescia, N.G.A.,Grimes, J.M.,Kivela, H.M.,Assenberg, R.,Sutton, G.C.,Butcher, S.J.,Bamford, J.K.H.,Bamford, D.H.,Stuart, D.I.
Insights Into Virus Evolution and Membrane Biogenesis from the Structure of the Marine Lipid-Containing Bacteriophage Pm2
Mol.Cell, 31:749-, 2008

PubMed Abstract: Recent, primarily structural observations indicate that related viruses, harboring no sequence similarity, infect hosts of different domains of life. One such clade of viruses, defined by common capsid architecture and coat protein fold, is the so-called PRD1-adenovirus lineage. Here we report the structure of the marine lipid-containing bacteriophage PM2 determined by crystallographic analyses of the entire approximately 45 MDa virion and of the outer coat proteins P1 and P2, revealing PM2 to be a primeval member of the PRD1-adenovirus lineage with an icosahedral shell and canonical double beta barrel major coat protein. The view of the lipid bilayer, richly decorated with membrane proteins, constitutes a rare visualization of an in vivo membrane. The viral membrane proteins P3 and P6 are organized into a lattice, suggesting a possible assembly pathway to produce the mature virus.

PubMed: 18775333
DOI: 10.1016/J.MOLCEL.2008.06.026

Experimental method

X-RAY DIFFRACTION (7 Å)