2VL8

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF LETHAL TOXIN FROM CLOSTRIDIUM SORDELLII IN COMPLEX WITH UDP, CASTANOSPERMINE AND CALCIUM ION

2VL8 の概要

• エントリーDOI: 10.2210/pdb2vl8/pdb
• 関連するPDBエントリー: 2VKD 2VKH
• 分子名称: CYTOTOXIN L, URIDINE-5'-DIPHOSPHATE, CASTANOSPERMINE, ... (5 entities in total)
• 機能のキーワード: toxin, glycosyltransferase
• 由来する生物種: CLOSTRIDIUM SORDELLII
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 192800.67

構造登録者

Jank, T.,Ziegler, M.O.P.,Schulz, G.E.,Aktories, K. (登録日: 2008-01-09, 公開日: 2008-06-17, 最終更新日: 2024-05-08)

主引用文献

Jank, T.,Ziegler, M.O.P.,Schulz, G.E.,Aktories, K.
Inhibition of the Glucosyltransferase Activity of Clostridial Rho/Ras-Glucosylating Toxins by Castanospermine.
FEBS Lett., 582:2277-, 2008

PubMed Abstract: Castanospermine was identified as an inhibitor of the Rho/Ras-glucosylating Clostridium sordellii lethal toxin and Clostridium difficile toxin B. Microinjection of castanospermine into embryonic bovine lung cells prevented the cytotoxic effects of toxins. The crystal structure of the glucosyltransferase domain of C. sordellii lethal toxin in complex with castanospermine, UDP and a calcium ion was solved at a resolution of 2.3A. The inhibitor binds in a conformation that brings its four hydroxyl groups and its N-atom almost exactly in the positions of the four hydroxyls and of the ring oxygen of the glucosyl moiety of UDP-glucose, respectively.

PubMed: 18505687
DOI: 10.1016/J.FEBSLET.2008.05.025

実験手法

X-RAY DIFFRACTION (2.31 Å)