2VJX

Structural and biochemical evidence for a boat-like transition state in beta-mannosidases

Summary for 2VJX

Related2VQT 2VOT 2VL4 2VQU 2VO5 2JE8 2VJX 2VMF
DescriptorBETA-MANNOSIDASE, (3S,4R,5R)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)PIPERIDIN-2-ONE, 1,2-ETHANEDIOL, ... (6 entities in total)
Functional Keywordslinear free energy relationship, hydrolase, transition state mimic, mannosidase, glycoside hydrolase
Biological sourceBACTEROIDES THETAIOTAOMICRON
Total number of polymer chains2
Total molecular weight200141.66
Authors
Tailford, L.E.,Offen, W.A.,Smith, N.L.,Dumon, C.,Moreland, C.,Gratien, J.,Heck, M.P.,Stick, R.V.,Bleriot, Y.,Vasella, A.,Gilbert, H.J.,Davies, G.J. (deposition date: 2007-12-14, release date: 2008-04-01, Last modification date: 2011-07-13)
Primary citation
Tailford, L.E.,Offen, W.A.,Smith, N.L.,Dumon, C.,Moreland, C.,Gratien, J.,Heck, M.P.,Stick, R.V.,Bleriot, Y.,Vasella, A.,Gilbert, H.J.,Davies, G.J.
Structural and Biochemical Evidence for a Boat-Like Transition State in Beta-Mannosidases.
Nat.Chem.Biol., 4:306-, 2008
PubMed: 18408714 (PDB entries with the same primary citation)
DOI: 10.1038/NCHEMBIO.81
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.85 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.20140.2%0.9%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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More Biological unit images

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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (2vjx.pdb1.gz [157.75 KB])
Coordinate files for Biological unit (2vjx.pdb2.gz [155.91 KB])