2VCO

Crystal structure of the fimbrial adhesin FimH in complex with its high-mannose epitope

Summary for 2VCO

• Entry DOI: 10.2210/pdb2vco/pdb
• Related: 1KIU 1KLF 1QUN 1TR7 1UWF 1ZE3
• Descriptor: PROTEIN FIMH, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, NICKEL (II) ION, ... (5 entities in total)
• Functional Keywords: pili, glycan, mannose, cell adhesion
• Biological source: ESCHERICHIA COLI
• Total number of polymer chains: 2
• Total formula weight: 35868.75

Authors

Wellens, A.,Garofalo, C.,Nguyen, H.,Wyns, L.,De Greve, H.,Hultgren, S.J.,Bouckaert, J. (deposition date: 2007-09-26, release date: 2008-05-13, Last modification date: 2024-10-23)

Primary citation

Wellens, A.,Garofalo, C.,Nguyen, H.,Van Gerven, N.,Slattegard, R.,Hernalsteens, J.P.,Wyns, L.,Oscarson, S.,De Greve, H.,Hultgren, S.J.,Bouckaert, J.
Intervening with Urinary Tract Infections Using Anti-Adhesives Based on the Crystal Structure of the Fimh-Oligomannose-3 Complex.
Plos One, 3:E2040-, 2008

PubMed Abstract: Escherichia coli strains adhere to the normally sterile human uroepithelium using type 1 pili, that are long, hairy surface organelles exposing a mannose-binding FimH adhesin at the tip. A small percentage of adhered bacteria can successfully invade bladder cells, presumably via pathways mediated by the high-mannosylated uroplakin-Ia and alpha3beta1 integrins found throughout the uroepithelium. Invaded bacteria replicate and mature into dense, biofilm-like inclusions in preparation of fluxing and of infection of neighbouring cells, being the major cause of the troublesome recurrent urinary tract infections.

PubMed: 18446213
DOI: 10.1371/JOURNAL.PONE.0002040

Experimental method

X-RAY DIFFRACTION (2.1 Å)