2VAL

Crystal structure of an Escherichia coli tRNAGly microhelix at 2.0 Angstrom resolution

Summary for 2VAL

• Entry DOI: 10.2210/pdb2val/pdb
• Descriptor: 5'-R(*GP*CP*GP*GP*GP*AP*AP)-3', 5'-R(*UP*UP*CP*CP*CP*GP*CP)-3', MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: rna, glycyl-trna synthetase (glyrs) system, trnagly acceptor stem microhelix, rna trna identity elements, magnesium binding sites
• Biological source: ESCHERICHIA COLI; More
• Total number of polymer chains: 4
• Total formula weight: 8889.84

Authors

Forster, C.,Brauer, A.B.E.,Perbandt, M.,Lehmann, D.,Furste, J.P.,Betzel, C.,Erdmann, V.A. (deposition date: 2007-09-03, release date: 2007-10-16, Last modification date: 2024-05-08)

Primary citation

Forster, C.,Brauer, A.B.E.,Perbandt, M.,Lehmann, D.,Furste, J.P.,Betzel, C.,Erdmann, V.A.
Crystal Structure of an Escherichia Coli Trnagly Microhelix at 2.0 Angstrom Resolution
Biochem.Biophys.Res.Commun., 363:621-, 2007

PubMed Abstract: tRNA identity elements determine the correct aminoacylation by the cognate aminoacyl-tRNA synthetase. In class II aminoacyl tRNA synthetase systems, tRNA specificity is assured by rather few and simple recognition elements, mostly located in the acceptor stem of the tRNA. Here we present the crystal structure of an Escherichia coli tRNA(Gly) aminoacyl stem microhelix at 2.0 A resolution. The tRNA(Gly) microhelix crystallizes in the space group P3(2)21 with the cell constants a=b=35.35 A, c=130.82 A, gamma=120 degrees . The helical parameters, solvent molecules and a potential magnesium binding site are discussed.

PubMed: 17888869
DOI: 10.1016/J.BBRC.2007.09.008

Experimental method

X-RAY DIFFRACTION (2 Å)