2V5Q

CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN

2V5Q の概要

• エントリーDOI: 10.2210/pdb2v5q/pdb
• 関連するPDBエントリー: 1Q4K 1Q4O 1UMW
• 分子名称: SERINE/THREONINE-PROTEIN KINASE PLK1, DESIGN ANKYRIN REPEAT PROTEIN (3 entities in total)
• 機能のキーワード: design ankyrin repeat protein, transferase complex, phosphorylation, nucleotide-binding, serine/threonine-protein kinase, kinase, nucleus, transferase, atp-binding, serine/threonine protein kinase
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 107617.68

構造登録者

Bandeiras, T.M.,Hillig, R.C.,Matias, P.M.,Eberspaecher, U.,Fanghaenel, J.,Thomaz, M.,Miranda, S.,Crusius, K.,Puetter, V.,Amstutz, P.,Gulotti-Georgieva, M.,Binz, H.K.,Holz, C.,Schmitz, A.A.P.,Lang, C.,Donner, P.,Egner, U.,Carrondo, M.A.,Mueller-Tiemann, B. (登録日: 2007-07-08, 公開日: 2008-04-01, 最終更新日: 2023-12-13)

主引用文献

Bandeiras, T.M.,Hillig, R.C.,Matias, P.M.,Eberspaecher, U.,Fanghanel, J.,Thomaz, M.,Miranda, S.,Crusius, K.,Putter, V.,Amstutz, P.,Gulotti-Georgieva, M.,Binz, H.K.,Holz, C.,Schmitz, A.A.,Lang, C.,Donner, P.,Egner, U.,Carrondo, M.A.,Muller-Tiemann, B.
Structure of wild-type Plk-1 kinase domain in complex with a selective DARPin.
Acta Crystallogr. D Biol. Crystallogr., 64:339-353, 2008

PubMed Abstract: As a key regulator of mitosis, the Ser/Thr protein polo-like kinase-1 (Plk-1) is a well validated drug target in cancer therapy. In order to enable structure-guided drug design, determination of the crystal structure of the kinase domain of Plk-1 was attempted. Using a multi-parallel cloning and expression approach, a set of length variants were identified which could be expressed in large amounts from insect cells and which could be purified to high purity. However, all attempts to crystallize these constructs failed. Crystals were ultimately obtained by generating designed ankyrin-repeat proteins (DARPins) selective for Plk-1 and using them for cocrystallization. Here, the first crystal structure of the kinase domain of wild-type apo Plk-1, in complex with DARPin 3H10, is presented, underlining the power of selective DARPins as crystallization tools. The structure was refined to 2.3 A resolution and shows the active conformation of Plk-1. It broadens the basis for modelling and cocrystallization studies for drug design. The binding epitope of 3H10 is rich in arginine, glutamine and lysine residues, suggesting that the DARPin enabled crystallization by masking a surface patch which is unfavourable for crystal contact formation. Based on the packing observed in the crystal, a truncated DARPin variant was designed which showed improved binding characteristics.

PubMed: 18391401
DOI: 10.1107/S0907444907068217

実験手法

X-RAY DIFFRACTION (2.3 Å)