2V50

The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB

2V50 の概要

• エントリーDOI: 10.2210/pdb2v50/pdb
• 分子名称: MULTIDRUG RESISTANCE PROTEIN MEXB, DODECYL-BETA-D-MALTOSIDE (2 entities in total)
• 機能のキーワード: multidrug resistance protein, ddm, rnd, membrane, detergent, transport, cell membrane, transmembrane, membrane protein, cell inner membrane, antibiotic resistance, drug-efflux pump, transport protein
• 由来する生物種: PSEUDOMONAS AERUGINOSA PA01
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P52002
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 686320.97

構造登録者

Sennhauser, G.,Bukowska, M.A.,Gruetter, M.G. (登録日: 2008-10-01, 公開日: 2009-05-19, 最終更新日: 2023-12-13)

主引用文献

Sennhauser, G.,Bukowska, M.A.,Briand, C.,Gruetter, M.G.
Crystal Structure of the Multidrug Exporter Mexb from Pseudomonas Aeruginosa.
J.Mol.Biol., 389:134-, 2009

PubMed Abstract: We report here the crystal structure of the Pseudomonas aeruginosa multidrug exporter MexB, an intensively studied member of the resistance-nodulation-cell division family of secondary active transporters, at 3.0 A. MexB forms an asymmetric homotrimer where each subunit adopts a different conformation representing three snapshots of the transport cycle similar to the recently determined structures of its close homologue AcrB from Escherichia coli, so far the sole structurally characterized member of the superfamily. As for AcrB, the conformations of two subunits can be clearly assigned to either the binding step or the extrusion step in the transport process. Unexpectedly, a remarkable conformational shift in the third subunit is observed in MexB, which has potential implications for the assembly of the tripartite MexAB-OprM drug efflux system. Furthermore, an n-dodecyl-d-maltoside molecule was found bound to the internal multidrug-binding cavity, which might indicate that MexB binds and transports detergent molecules as substrates. As the only missing piece of the puzzle in the MexAB-OprM system, the X-ray structure of MexB completes the molecular picture of the major pump mediating intrinsic and acquired multidrug resistance in P. aeruginosa.

PubMed: 19361527
DOI: 10.1016/J.JMB.2009.04.001

実験手法

X-RAY DIFFRACTION (3 Å)